Protein A  Magnetic BeadsProtein G Magnetic BeadsProtein L Magnetic  BeadsProtein A/G Magnetic BeadsProtein A  Agarose BeadsProtein G  Agarose BeadsProtein L  Agarose BeadsProtein A/G  Agarose BeadsAnti-HA Magnetic BeadsAnti-Myc  Magnetic BeadsAnti-Flag  Magnetic BeadsStreptavidin Magnetic BeadsAnti-Flag  Affinity  GelAnti-GST Magnetic BeadsAnti-His Magnetic BeadsAnti-GFP Magnetic BeadsConA Magnetic Beads免疫沉淀磁珠His蛋白纯化琼脂糖凝胶GST蛋白纯化琼脂糖凝胶His蛋白纯化琼脂糖磁珠GST蛋白纯化琼脂糖磁珠Protein A琼脂糖磁珠Protein G琼脂糖磁珠Protein A/G琼脂糖磁珠抗体纯化磁珠PCR产物提取磁珠oligo-dT磁珠核酸提取磁珠羟基磁珠氨基磁珠羧基磁珠醛基磁珠NHS磁珠硅基磁珠Protein A IP/Co-IP KitProtein G IP/Co-IP KitClassic IP/Co-IP KitHA IP/Co-IP KitMyc IP/Co-IP KitFlag  IP/Co-IP KitFlag IP/Co-IP Kit(凝胶)GST IP/Co-IP KitHis IP/Co-IP KitGFP IP/Co-IP KitBasic IP/Co-IP KitHis-Tag Pulldown KitGST-Tag Pulldown Kit免疫沉淀试剂盒His-Tag蛋白纯化试剂盒GST-Tag蛋白纯化试剂盒蛋白纯化试剂盒生物配体快速偶联试剂盒生物配体快速偶联试剂盒外泌体免疫沉淀试剂盒尿液外泌体提取试剂盒细胞上清外泌体提取试剂盒血清外泌体提取试剂盒血浆外泌体提取试剂盒其他体液外泌体提取试剂盒外泌体研究产品双排4孔 1.5mL磁力架双排8孔 1.5mL磁力架双排16孔 1.5mL磁力架双排4孔 15mL磁力架双排4孔 50mL磁力架手持均质仪配套设备生物偶联技术高通量蛋白纯化外泌体定向改造IVD试剂研发服务磁珠应用外泌体专题纳米抗体神经科学领域新冠相关PROTAC技术翎因动态行业新闻优惠促销产品支持技术支持客户发表文章学习资源企业简介企业文化团队风采生产与质量联系我们
Lab  on  the Beads
ConA Magnetic Beads

ConA Magnetic Beads

货号 L-1017/L-1017A
规格 1mL/5mL
价格 ¥ 999/3999

Product Description

Bead- and column-based separation methods relyheavily on the speed and ease of affinity binding systems. Ligands such asstreptavidin, antibodies and lectins are used both to capturespecifically-tagged targets and for the isolation of cells and biomoleculesthat naturally express the ligand binding partner. The uniquesaccharide-binding properties of plant lectins, such as Concanavalin A (Con A)have made them useful for the labeling and isolation of glycan presenting cellsand glycoproteins in serum and cell lysate. Lectins have additionally been usedin cell adhesion studies, to effect lymphocyte activation, and to explorecarbohydrate-based therapeutics. Concanavalin A magnetic beads has also beenused for CUT&RUN, a chromatin profiling protocol that has several keyadvantages over ChIP.

Product Features


Concanavalin A



Particle size



10 mg/mL


Isolating glycoproteins,CUT&RUN,

Storage Condition

Store at 4°C for 2 years.


Researchers are advised tooptimize use of beads in any application

Material Required

1.5mL or 2mL microcentrifugetubes

Mammalian cells(10000-100000cells)

Binding Buffer:1x PBS + 1mM MgCl2 + 1mM MnCl2+

1mM CaCl2 (pH7.4)

Wash Buffer:1x PBS + 1mM MgCl2 + 1mM MnCl2+ 1mM CaCl2 (pH 7.4) + 0.1% Tween® 20

Elution Buffer;5mM Tris (pH 8.0) + 0.15M NaCl + 0.05% SDS + 1MGlucose

Preparation of Cells

1.Prepare mammalian cells10000-100000 cells)sample,Centrifuge at 600 x g for 3-5 minuteat room temperature., Carefully remove the supernatant

2. Add 90ul of BindingBuffer to cells, Mix the tube to resuspend the cells. Centrifugeat 600 x g for 3-5 minute at room temperature, carefully remove the supernatant

3. Add 90μL of ElutionBuffer to the cells and resuspend carefully.

Preparation of Magnetic Beads

4. Transfer 10uL of Con A MagneticBeads to a clean microcentrifuge tube. Place the tube on a magnet to separatethe beads from solution.Carefully removeand discard the solution.

5. Wash the beads byadding 200μL of Binding Buffer. Mix well.   

6. Repeat the particlewash 1 more time. After the last wash, remove the supernatant.

7. Add the cells sample from Step 3 to the beadsand mix well by inversion to resuspend the beads.

8. Place the sample on a tube rotator and mixfor 10-30 minutes at room temperature.

9. Remove the sample from the rotator and placein a magnetic separator. Carefully remove the cleared supernatant.

10. Wash the beads by adding 0.5mL of WashBuffer. Mix well by inversion or by vortex mixing.

11. Repeat Steps 9-10. Resuspend the beads with0.5mL of Wash Buffer and place on tube rotator for 5 minutes.

12. Repeat Steps 9-10.

13. Replace the tube of beads on the magneticseparator and carefully remove / discard the supernatant.

14. Add 50-250μL ofElution Buffer to the beads. Mix the tube to resuspendthe beads and place the tube on rotator for 10-30 minutes at room temperature.

15. Replace the tube of beads on the magneticseparator and carefully remove the eluate and transfer to a cleanmicrocentrifuge tube for later use or storage.

16. Repeat Steps 14-15. Eluates may be pooledand precipitated. Store eluates on ice for immediate use or freeze forlong-term storage.


• Avoid the use of reagentswith EDTA or other metal chelators, as this will reduce the effectiveness ofthe Binding Buffer.

•Protease Inhibitors may beused when sensitive glycoproteins are isolated.

•Low glycoprotein recovery may be attached by either increasing the elutionincubation time beyond 10 minutes, and / or by boiling beads in 200μL of SDS-PAGEsample buffer for 5 minutes and then magnetically separating the beads from theeluate. (Note: Boiling may detach some lectins and may also release nonspecificallybound proteins.


ConA Magnetic Beads产品说明书