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企业简介企业文化团队风采生产与质量Protein A  Magnetic BeadsProtein G Magnetic BeadsProtein L Magnetic  BeadsProtein A/G Magnetic BeadsProtein A  Agarose BeadsProtein G  Agarose BeadsProtein L  Agarose BeadsProtein A/G  Agarose BeadsAnti-HA Magnetic BeadsAnti-Myc  Magnetic BeadsAnti-Flag  Magnetic BeadsStreptavidin Magnetic BeadsAnti-Flag  Affinity  GelAnti-GST Magnetic BeadsAnti-His Magnetic BeadsAnti-GFP Magnetic BeadsConA Magnetic Beads免疫沉淀磁珠His蛋白纯化琼脂糖凝胶GST蛋白纯化琼脂糖凝胶His蛋白纯化琼脂糖磁珠GST蛋白纯化琼脂糖磁珠Protein A琼脂糖磁珠Protein G琼脂糖磁珠Protein A/G琼脂糖磁珠抗体纯化磁珠PCR产物提取磁珠oligo-dT磁珠DNA Normalizer Magnetic Beads核酸提取磁珠羟基磁珠氨基磁珠羧基磁珠醛基磁珠NHS磁珠硅基磁珠Protein A IP/Co-IP KitProtein G IP/Co-IP KitClassic IP/Co-IP KitHA IP/Co-IP KitMyc IP/Co-IP KitFlag  IP/Co-IP KitFlag IP/Co-IP Kit(凝胶)GST IP/Co-IP KitHis IP/Co-IP KitGFP IP/Co-IP KitBasic IP/Co-IP KitHis-Tag Pulldown KitGST-Tag Pulldown Kit免疫沉淀试剂盒His-Tag蛋白纯化试剂盒GST-Tag蛋白纯化试剂盒蛋白纯化试剂盒生物配体快速偶联试剂盒生物配体快速偶联试剂盒外泌体免疫沉淀试剂盒尿液外泌体提取试剂盒细胞上清外泌体提取试剂盒血清外泌体提取试剂盒血浆外泌体提取试剂盒其他体液外泌体提取试剂盒外泌体研究产品双排4孔 1.5mL磁力架双排8孔 1.5mL磁力架双排16孔 1.5mL磁力架双排4孔 15mL磁力架双排4孔 50mL磁力架手持均质仪配套设备生物偶联技术高通量蛋白纯化外泌体定向改造IVD试剂研发服务翎因动态行业新闻
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产品详情
Anti-Flag  Affinity  Gel
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Anti-Flag  Affinity  Gel
货号: L-1013/L-1013A
规格: 1mL/5mL
价格: ¥ 1999/7999
产品详情

Product Description

Anti-Flag Affinity Gel are based on sepharose 4B, with 90um particle size, covalently coupling with high quality mouse IgG monoclonal antibody that recognizes the Flag-epitope tag (DYKDDDDK).

Product Features

Composition

mouse   IgG monoclonal Ab

Matrix

4% cross-linked agarose

Particle size

90 um

Concentration

50% settled resin in TBS with 0.02% sodium azide

Binding Capacity

1 mg Flag-tagged   fusion protein/mL of settled resin

Application

rProtein Purification,IP

Storage Condition

Store at 4°C for 2 years.

Protocol

Isolation of Flag fusion protein with Anti-Flag Affinity Gel

1. Thoroughly suspend Anti-Flag Affinity Gel in the vial, in order to make a uniform suspension ofthe resin. Quickly transfer 10μL of the gel suspension (about 5μL of packed gel volume) to a fresh tube.

2.   Add 0.6mL TBS. Thoroughly suspend the Anti-Flag Affinity Gel. Centrifuge the resin at 10000 rpm for 30 seconds. Remove the supernatant carefully. Be sure that most of the wash buffer is removed and no resin is discarded. The step should be repeated for 3-4 times.

3.   Add 500μL of E.coli periplasmicextracts to the washed resin.

4.   Agitate all samples gently for 2 hour at 4°C.

5.   Centrifuge the resin for 30 seconds at10000 rpm. Remove the supernatants to a fresh tube.

6.   Wash the resin with 0.5ml TBS until the OD280 of the supernatant liquid<0.05.

7.   Add 10μL of 2x sample buffer to each sample(the resin and the supernatant). Boil the sample for 5 minutes.

8.   Load 4μL of each sample on SDS-PAGE.


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Anti-Flag   Affinity   Gel‍说明书




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